
Interpreting Feed & Forage Analysis
As Received
Dry Basis
Crude Protein
Heat Damaged Protein
Unavailable Protein
Available Protein
Soluble Protein
Acid Detergent Fiber (ADF)
Neutral Detergent Fiber (NDF)
Total Digestible Nutrients (TDN)
Net Energy-Lactation, Net Energy-Maintenance
and Net Energy-Gain
Relative Feed Value (RFV)
Relative Feed Quality (RFQ)
Minerals
Fat
Ash
Lignin
Starch
Neutral Detergent Fiber
Digestibility (NDFd)
Volatile Fatty Acids (VFA)
Yeast/Mold Count
Protein Dispersibility Index
(PDI)
As Received
All values under this heading show the content of the
nutrients with the moisture in the sample included.
Because of the dilution of water, the values will be lower than
the dry matter basis.
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Dry Basis
Values in this column give the nutrient values with the water
removed. This allows comparisons to be made between
forages. This is the best indicator of nutrient value
because forages are evaluated on a dry matter basis.
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Crude Protein
The measure of the nitrogen content of a forage is used to
calculate the crude protien content as follows: CP = %N x
6.25. Crude Protein will include both true protein and
non-protein nitrogen. Crude Protein values give no
indication if heat damage has occurred.
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Heat Damaged Protein
The measure of protein determines if a % was damaged or bound
due to excessive heat. This is most commonly found in
haylages.
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Unavailable Protein
A forage report will not always show this value unless heat
damage has occurred. This value will give an indication if
excessive heating has occurred, reducing the protein
digestibility. All forages have some unavailable protein.
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Available Protein
In some reports this value will be the difference between the
crude protein and unavailable protein value. However, some
laboratories account for the naturally bound protein found in
forages.
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Soluble Protein
This is protein which is rapidly degraded to ammonia in the
rumen and reported as % of crude protein. Some of this
rapidly available protein is needed by the rumen bacteria when
their growth is high.
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Acid Detergent Fiber (ADF)
The ADF value refers to the cell wall portions of the forage
that are made up of cellulose and lignin. These values are
important because they relate to the ability of an animal to
digest the forage. As ADF increases the ability to digest
or the digestibility of the forage decreases.
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Neutral Detergent Fiber (NDF)
The NDF value is the total cell wall which is comprised ot
the ADF fraction plus hemicellulose. NDF values are
important because they reflect the amount of forage the animal
can consume. As NDF percent increases, the dry matter
intake generally decreases.
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Total Digestible Nutrients (TDN)
Some laboratories use the same formula to calculate the TDN
value as they do the Digestible Dry Matter (DDM); therefore, the
two values would be the same. Other laboratories will use
different formulas, such as:
Alfalfa: % TDN = 96.35 - (ADF
% x
.15)
Corn Silage: % TDN = 87.84 - (ADF
% x 0.70)
As the percent ADF increases, TDN will decrease.
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Net Energy-Lactation, Net Energy-Maintenance and Net
Energy-Gain
These net energy values are often calculated from TDN values,
which in turn are generated from percent ADF. Examples
are:
NEL: Mcal/lb = (TDN % x
0.01114) - 0.054
NEM: Mcal/lb = (TDN % x
0.01318) - 0.132
NEG: Mcal/lb = (TDN % x
0.01318) - 0.459
As the percent ADF in the forage increases, the net energy
values will decrease.
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Relative Feed Value (RFV)
Relative feed value is an index that combines the important
nutritional factors of intake and digestibility. It has no
units, but the index allows for comparisons of legume, grass and
legume/grass forages. A relative feed value is not to be
used for nutritional ration balancing. Instead, it will
assist in determining the value of a material for marketing
purposes.
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Relative Feed Quality (RFQ)
Relative feed quality is calculated by estimating the
digestibility of the forage dry matter and by determining how
much the cow can eat based on its filling capacity. The
RFQ index uses fiber digestibility to estimate intake as well as
the total digestible nutrients (energy) of the forage.
This can be used to more accurately predict the animals'
performance and match the animals needs.
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Minerals
Wet chemistry processes determine macro minerals as percent
and trace minerals as parts per million (ppm). If the Near
Infrared Spectroscopy (NIRS) is used, the test will include only
the macro minerals. The macro minerals include percent
calcium, phosphorus, potassium and magnesium. The use of
wet chemistry will if requested include iron as ppm, copper,
zinc and manganese.
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Fat
These are the oils and related compounds found in grains and
forages that naturally make-up approximately 2-4 percent of the
ration.
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Ash
This represents the mineral content of the feed or
forage. Forages contain 3 to 12 percent ashes on a dry
weight basis while grains and concentrates contain 1 to 4
percent.
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Lignin
Lignin is the prime factor influencing the digestibility of
plant cell wall material. As lignin increases, the
digestibility, intake and animal performance usually
decreases. As the percent of lignin increases, the
percentage of ADF and NDF also increases in forage.
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Starch
Starch is classified as a carbohydrate or non-structural in
definition. Carbohydrates reside within the cell or
contents and their digestibility is about 90 percent.
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Neutral Detergent
Fiber Digestibility (NDFd)
NDFd is a measure of the
digestibility of neutral detergent fiber. This approach
enables nutritionists to formulate better rations. More
accurate energy predictions can be made and dry matter intake
achieved. Both wet In vitro and NIRS tests are available.
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Volatile Fatty Acids
(VFA)
Fermentation analysis will
assist in understanding the quality of the fermentation and a
silage. Data interpreted will tell us whether an excellent,
average or poor fermentation occurred. An analysis report will
include pH, lactic, acetic, propionic and butyric acids, ammonia and
ethanol.
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Yeast/Mold Count
A yeast and mold count is
perhaps one of the most important tests when determining whether
microbial changes in a silage will degrade the nutritional content;
thus, leading to possible mycotoxin formation. Yeasts counts
are a good indicator of unstable or hot silage.
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Protein
Dispersibility Index (PDI)
The PDI test determines how
thoroughly soybeans have been heated to deactivate the trypsin
inhibitor. Heat causes a chemical reaction to occur between
protein and sugars resulting in a protein-sugar complex know as
Maillard Products. This in turn influences the rate of protein
being degraded in the rumen.
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